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Cross-sectional study of nutritive profile and association with feeding practices in infants at 6 to 7 months of age
*Corresponding author: Abdul Khader, Department of Neonatology, Ankura Hospital for Women and Child Health, Telangana, India. a.khaderkp@gmail.com
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Received: ,
Accepted: ,
How to cite this article: Khader A, Krishna V. Cross-sectional study of nutritive profile and association with feeding practices in infants at 6 to 7 months of age visiting tertiary care hospital. Karnataka Paediatr J. doi: 10.25259/KPJ_28_2026
Abstract
Objectives:
The objective of the study is to compare anthropometric indices and serum calcium and iron status among apparently healthy 6–7-month-old term infants across three feeding groups: Exclusive breastfeeding, early cessation of breastfeeding with exclusive formula feeding thereafter (non-breastfed) and continued breastfeeding with complementary feeds (early complementary feeding).
Material and Methods:
This descriptive cross-sectional study was conducted from November 2022 to June 2024 at a tertiary paediatric hospital. A total of 215 apparently healthy term infants aged 6–7 months attending the hospital for routine immunisation or well-baby follow-up visits were enrolled. Weight, length and head circumference were measured using standard techniques. Venous blood samples were collected for estimation of serum calcium and iron status. Anthropometric indices were expressed as the World Health Organization Z-scores.
Results:
There were no statistically significant differences among the three feeding groups with respect to length-for-age, weight-for-age, weight-for-length z-score categories, head circumference centiles, serum calcium levels or biochemical iron deficiency.
Conclusion:
Among apparently healthy term infants aged 6–7 months, different feeding practices were associated with comparable anthropometric measurements and biochemical markers of calcium and iron status.
Keywords
Complementary feeding
Exclusive breastfeeding
Infant feeding practices
INTRODUCTION
Optimal nutrition in the first few months of life is critical for long-term growth and development. Health organisations throughout the globe, such as the World Health Organization (WHO), American Academy of Pediatrics (AAP), Indian Academy of Pediatrics (IAP), Infant and Young Child Feeding (IYCF), all recommend EBF for the first 6 months of life, followed by the introduction of complementary feeding while continuing breastfeeding till 2 years of age.[1] Despite these recommendations for 6 months of EBF, global trends show an increase in formula feeding and early initiation of complementary foods.
Infant feeding practices vary across the globe and have been rapidly changing owing to various social, economic and cultural changes. Some factors influencing infant feeding trends include maternal education, employment status and maternity leave duration for working mothers, conveniency for mother, familial supportive environment and access to health care. Sometimes, poor comprehension of EBF guidelines or conflicts with traditional beliefs and cultural practices lead to early interruption of EBF.[2] At times, inadequate communication and understanding about the adequacy and sufficiency of EBF may be the impediments.[3] Many baby formula feeds are being commercially marketed as ‘enriched and fortified’ with nutritional supplements. While fortification of pre-term formula feeds has been shown to be beneficial, there are limited studies in this regard for term infants. Despite the strong endorsement for breastfeeding, there has been an increased tendency to formula feed babies even in low and middle-income countries, where it adds to the financial burden of the household. Studies in many Western countries reveal that breastfeeding is no longer the major mode of feeding and most infants are started on some formula feeding before 2 months of age.[2] The Problem: In India, while EBF rates rose to 43% by 2019 National Family Health Survey-5 (NFHS-5), over 50% of the infant population still does not receive EBF for the full 6 months.[4,5] Although there has been a rise in the percentage of EBF babies, >50% of Indian infant population is still not exclusively breastfed. In a large survey carried out in the United States, while breastfeeding initiation rates were as high as 80%, the percentage of mothers who were receiving some breastfeeding at 6 and 12 months was only 27.0% and 12.3%, respectively, while EBF was only 7.9% at 6 months.[2] In another follow-up study in the U.S. in 2017, during the 1st month of life, while 53% mothers attempted to breastfeed, the largest category by the end of 1st month is of formula-fed babies (32.9%), followed bydirect breast feeding (DBF) (23.8%), followed by mixed feeding. Mothers who used formula feed were found to be less educated and were employed fulltime.[6,7]
Exclusive breastfed children have a lower incidence of otitis media, Upper Respiratory Tract Infections (URTI), Lower Respiratory Tract Infections (LRTI), asthma, Respiratory Syncitial Virus (RSV) bronchiolitis, Necrotizing EnteroColitis (NEC), atopic dermatitis, inflammatory bowel disease, obesity, celiac disease , Sudden Infant Death Syndrome (SIDS) and malignancies like leukaemia and better neurodevelopmental outcome compared to commercial formula-fed children. Better neurodevelopmental outcome was also found in infants, including pre-terms who were exclusively breastfed.[8,9] The window of vulnerability: The 6–7-month period is a vital transition from milk to solids, yet there is a lack of data on how varied feeding practices during this time correlate specifically with physical growth parameters and micronutrient deficiencies in healthy term infants.
Objectives
The primary goal was to compare anthropometric indices (weight, length and head circumference) and biochemical markers (serum calcium and iron status) among healthy 6–7-month-old term infants. The study compared three specific feeding groups:
EBF: Exclusively breastfed until 6 months
Non-breastfed (NBF): Formula feeding after breastfeeding was stopped before 3 months of age
ECF: Introduction of solids alongside breast milk or formula at any age.
MATERIAL AND METHODS
Study design
A descriptive cross-sectional study was conducted from November 2022 to June 2024 at a tertiary paediatric hospital.
Participants
A total of 215 healthy term infants aged 6–7 months were enrolled during routine immunisation or well-baby visits.
Measurements
Growth - Anthropometric indices were measured and expressed as WHO Z-scores
Biochemical: Venous blood samples were analysed for serum calcium and iron.
Exclusions
Preterm infants and those with chronic illnesses (e.g., malabsorption, celiac disease or major cardiac/renal disease) were excluded to ensure a healthy baseline.
RESULTS
Feeding distribution
Of the 215 infants, 63.7% were EBF, 24.2% were NBF and 12.1% were ECF.
Growth outcomes
There were no statistically significant differences across the three groups for length-for-age, weight-for-age, weight-for-length or head circumference.
Biochemical outcomes
Low serum calcium (<8 mg/dL) was found in 9.3% of infants
Low serum iron (<50 mug/dL) was found in 19.1% of infants
Neither deficiency showed a statistically significant association with any specific feeding practice (p > 0.05).
Summary
Nutritional status at 6–7 months remained preserved regardless of the feeding mode.
DISCUSSION
Consistency with literature
The study aligns with existing research showing varied growth trajectories in infancy; however, it diverges from some studies suggesting that formula feeding is strongly linked to rapid weight-for-length increases in early life.
The ‘protective’ nature of EBF
While growth indices were comparable, distributional trends still slightly favoured the EBF and ECF groups, consistent with the general understanding of breastfeeding’s protective health benefits.
Micronutrient risks
The authors noted that while their iron findings were comparable, the literature suggests that prolonged EBF beyond 4 months without timely complementary feeds can eventually increase the risk of iron deficiency.
Study limitations
The cross-sectional design prevents determining causality or long-term trends.
Potential bias – due to single-centre hospital sampling and observer bias in measurements may affect results.
Confounding factors – Socioeconomic status and maternal health were not adjusted for in the analysis.
What Is Already Known
The WHO endorses exclusive breastfeeding for the first 6 months of life, followed by the introduction of appropriate complementary feeds.
Feeding practices differ worldwide and are rapidly evolving. Global data show an increasing trend towards formula feeding, early discontinuation of breastfeeding and early initiation of complementary feeding.
Feeding practices during the complementary feeding window (around 6–7 months) are a known period of vulnerability for growth and infant nutritional status. However, suboptimal breastfeeding practices and formula feeding in infants under 6 months are not yet thoroughly associated with undernutrition and micronutrient deficiencies.
What This Study Adds
Preserved nutritional status at 6–7 months: Despite varied feeding practices, infants across all groups showed similar growth and biochemical profiles. No feeding mode was associated with clear anthropometric or biochemical deficits/benefits.
Favourable trends with exclusive breastfeeding (EBF)/ early complementary feeding (ECF): Distributional trends favoured exclusively breastfed and early complementary feeding groups, consistent with potential benefits.
Support for existing WHO recommendations.
CONCLUSION
Among healthy term infants aged 6–7 months, different feeding practices resulted in comparable physical growth and biochemical calcium/iron status.
The findings support the existing WHO recommendations by demonstrating that infants can maintain a normal nutritional profile at the start of the complementary feeding window, though further long-term follow-up is necessary to track health trajectories into childhood.
Ethical approval:
The research/study was approved by the Institutional Review Board at Osmania Medical College, approval number ECR/300/Inst/AP/2013/RR-19, dated 25th August 2022.
Declaration of patient consent:
The authors certify that they have obtained all appropriate patient consent forms. In the form, the patient has given consent for clinical information to be reported in the journal. The patient understands that the patient’s names and initials will not be published and due efforts will be made to conceal their identity, but anonymity cannot be guaranteed.
Conflicts of interest:
There are no conflicts of interest.
Use of artificial intelligence (AI)-assisted technology for manuscript preparation:
The authors confirm that they have used artificial intelligence (AI)-assisted technology (ChatGPT GO) solely for language refinement and to improve the clarity of writing. No AI assistance was employed in the generation of scientific content, data analysis or interpretation.
Financial support and sponsorship: Nil.
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